PrimerBank | Tool name | PrimerBank |
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| URL | http://pga.mgh.harvard.edu/primerbank/ |
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| Important features | 1. PrimerBank is a public resource of primers designed for gene expression detection or quantification using real-time PCR.
2. There are primers covering almost all genes of mouse and human.
3. Database is validated using 26,855 primer pairs for 27,681 genes of mouse. |
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| Citations | Xiaowei Wang, Athanasia Spandidos, Huajun Wang and Brian Seed: PrimerBank: a PCR primer database for quantitative gene expression analysis, 2012 update. Nucleic Acids Research. 2011 Oct 21;40(D1): D1144-D1149. PubMed PMID: 22086960; PubMed Central PMCID: PMC3245149 |
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| Year of publication | 2012 |
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| Rank by usage frequency | 98 |
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| Comments | There are two older version of this database. One is 2003 version and other is 2010 version |
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| Function | SNP primers, Real time PCR, General PCR, Primer Database |
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| Category | Online, Free |
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| Features | 1. It is a database that holds primers generated for almost all genes of mouse and human.
2. Database is validated using 26,855 primer pairs for 27,681 genes of mouse using real time PCR followed by agarose gel electrophresis and sequencing PCR product. Success rate of this validation is 82.6% (22,187).
3. There are 129,692 primer pairs for human and 118,886 primer pairs for mouse. |
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